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Impaired chromatin remodelling at STAT1-regulated promoters leads to global unresponsiveness of Toxoplasma gondii -infected macrophages to IFN-gamma. 2012 Lang C, Hildebrandt A, Brand F, Opitz L, Dihazi H, Luder CG. Toxoplasma gondii prevents chromatin remodelling initiated by TLR-triggered macrophage activation. 2009 Leng J, Butcher BA, Egan CE, Abdallah DS, Denkers EY. Toxoplasma gondii inhibits covalent modification of histone H3 at the IL-10 promoter in infected macrophages. It can influence the host’s transcriptome, including through modulation of the host epigenome ( Leng and Denkers 2009 Leng J, Denkers EY. Toxoplasma gondii is a ubiquitous eukaryotic parasite associated with congenital or acquired ocular and/or brain lesions. Toxoplasma gondii nuclear localisation epigenetics

The pipeline is freely available online, uses open access tools and software with user-friendly Perl scripts to automate and manage the results, and is readily adaptable to undertake any such in silico search for genes contributing to particular functions. This identified 57 of the 611 putative epigenetic proteins as likely to be secreted.

Step 3 filtered for secretory proteins using SignalP, SecretomeP, and experimental data. This resulted in 611 putative nuclear localised proteins with predicted epigenetic functions.

Step 2 uses GOLink to identify proteins of epigenetic function based on Gene Ontology terms. Step 1 employs PredictNLS and NucPred to identify genes predicted to target eukaryotic nuclei. To identify genes with putative epigenetic functions, we developed an in silico pipeline to interrogate the T. Toxoplasma gondii uses epigenetic mechanisms to regulate both endogenous and host cell gene expression.